Durham Research Center, University of Nebraska Medical Center, Omaha
2005 – 2010 Ph.D. (Major:
Pharmaceutical Sciences, Minor: Protein Brain Delivery) University of
Nebraska Medical Center (UNMC), Omaha,
Alexander V. Kabanov
1995 - 1999 B. S. in Pharmaceutical
Sciences – Peking University Health Science Center, Beijing,
I am a 5rd year graduate student of
Pharmaceutical Sciences Graduate Program. I am working on protein
modification with a block polymer Pluronic to enhance protein
intracellular delivery and brain delivery. Protein drugs are potential
pharmaceutical agents for the treatment of many central nervous system (CNS)
disorders. However, they generally do not cross the blood-brain barrier (BBB)
due to poor lipophilicity and/or large molecular mass and/or absence of
specialized BBB receptor-mediated transport system. In some other cases,
penetration of neuronal cell membranes is crucial for proteins to exert the
function and provide therapeutic efficacy. My work is to synthesize
Pluronic-protein conjugates using degradable or non-degradable linkers,
characterize the products using various methods including MALDI-TOF, SDS-PAGE,
IEF, WB, ELISA, SPR, AFM and activity assays, and determine the Pluronic-protein
intracellular delivery and brain delivery in vitro and in vivo. In particular, I
have been working in the following three projects:
I. Horseradish peroxidase
(HRP) modification by Pluronic for enhanced uptake in and transport across the
bovine brain microvessel endothelial cell monolayers
II. Pluronic-Modified Superoxide Dismutase 1 (SOD1) Attenuates
Angiotensin II-Induced Increase in Intracellular Superoxide in
III. Pluronic modified leptin to across the BBB and for the
treatment of obesity.
• NanoDDS’09 Poster Award for
Outstanding Scientific Poster Display and Oral Presentation, Nanomedicine and
Drug Delivery Symposium (NanoDDS’ 09) 2009
• American Heart Association
(AHA) Midwest Predoctoral Fellowship 2009-2011
• UNMC Graduate
• Servier Young Investigator Award in Medicinal
Chemistry, Institute de Recherches Internationales Servier, 2003
Huang Zhou Yu Young Investigator Award, Institute of Materia Medica
• Outstanding Undergraduate Award, Peking University
Health Science Center, 1999
• Excellent Undergraduate Thesis Award,
Peking University Health Science Center, 1999
• Excellent Academic
Scholarship, Peking University Health Science Center,
• Yi, X., Zimmerman,
M.C., Yang, R.; Tong, J., Vinogradov, S., Kabanov, A.V. Pluronic-Modified
Superoxide Dismutase 1 (SOD1) Attenuates Angiotensin II-Induced Increase in
Intracellular Superoxide in Neurons. Free Radical Biology &
Medicine. 2010. Accepted.
• Price TO, Farr SA, Yi X, Vinogradov S,
Batrakova EV, Banks WA, Kabanov AV. Transport Across the Blood-Brain Barrier of
Pluronic Leptin. J Pharmacol Exp Ther. 2010 Apr; 333(1):253-63.
• Yi, X., Batrakova, E., Banks, W. A., Vinogradov, S., and Kabanov, A.
V. Protein Conjugation with Amphiphilic Block Copolymers for Enhanced Cellular
Delivery. Bioconjug Chem. 2008 May;19(5):107.
• Gilmore, J. L., Yi, X.,
Quan, L., and Kabanov, A. V. Novel Nanomaterials for Clinical Neuroscience.
J Neuroimmune Pharmacol. 2008 Jun;3(2):83. Equal contribution for the
first and second author.
• Wenhui Hu, Zongru Guo, Xiang Yi, Changbin
Guo, Fengming Chu, Guifang Cheng. Discovery of 2-Phenyl-3-sulfonylphenyl-indole
Derivatives as a New Class of Selective COX-2 Inhibitors. Bioorganic &
Medicinal Chemistry. 2003 Vol(11):5539.
• Changbin Guo, Xiang Yi,
Zongru Guo and Zhibing Xu. The Study on Molecular Mechanism and COX-2
Selectivity of Flurbiprofen Derivative, Acta Chimica Sinica, 2003 Vol
• Xiang Yi, Zongru. Guo, Feng Ming. Chu. Study on Molecular
Mechanism and 3D-QSAR of Influenza Neuraminidase Inhibitors. Bioorganic
& Medicinal Chemistry, 2003, Vol(11): 1465.
• Peicheng Zhang,
Yinghong Wang, Xin Liu, Xiang Yi, Ruoyun Chen, Dequan Yu. Conformation Study of
8-glucosyl-prunetin by Dynamic NMR Spectroscopy, Chinese Chemical
Letters, 2002, 13:645.
• Zongru Guo, Xiang Yi, Zhibing Xu.
Molecular Simulation of Interaction Between Estrogen Receptor and Selective
Estrogen Receptor Modulators. Acta Pharmacologica Sinica, 2002,
• LI Wei, YI Xiang, GUO Zong-Ru et al. 3D-QSAR Study of Wu
Wei Zi for Anti-AIDS Using Comparative Molecular Similarity Indices Analysis.
Acta Chimica Sinica, 2002, Vol 60: 1311.
• Shengb Wan, Xiang
Yi, Zongru Guo. Three Dimensional Quantitative Structure--Activity Relationship
of A Series of Benzocylohept-apyridine Farnesyl Transferase Inhibitors. Acta
Pharmaceutica Sinica, 2002,37:257.
• Xiang Yi, Zongru Guo. Three
Dimensional Quantitative Structure--Activity Relationship of PPARγ Agonists.
Acta Pharmaceutica Sinica, 2001, Vol 36: 262
• Zongru Guo,
Xiang Yi, Minmin Wang, Fengming Chu. Molecular Modeling and QSAR Studies on the
Interaction Mechanism of Retinoids Binding to RXR. Acta Chimica Sinica,
2001, Vol 59: 1925.
• Shengb Wan, Xiang Yi, Zongru Guo. Three
Dimensional Quantitative Structure--Activity Relationship of Farnesyl Protein
Transferase Inhibitors. Acta Pharmaceutica Sinica, 2001, Vol
• Jie Wang, Qiang Zhang, Xiang Yi, et al. Effect of Surface
Modification on the Uptake of Polylactic Acid Nanoparticles Loading Cyclosporine
A by Cells in vitro and on the Body Distribution in vivo. Journal of Beijing
Medical Univeristy, 2000, Vol 32:235.
What do you do when you
are not in the lab?
I enjoy my research very much because it fits my
curiosity about science and I have chance to meet interesting people in this
field. Outside of science, I like to travel, play volleyball and ping pong, and
Where are you from? How many languages
do you speak?